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MATLAB and Arduino C++ code for microfluidic DNA analysis systems

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Description

The advancement of multiple fields of science and engineering have allowed the development of lab-on-a-chip devices, capable of performing biological, chemical, and
other laboratory functions on a miniaturized device. The development of these type of devices are important in solving current medical and healthcare problems, such as the need for fast pathogen detection (i.e. ebola epidemic, HIV, water-borne diseases typhoid, cholera, dysentery) and the need for personalized medicine (i.e. cancer genomics, drug susceptibility). Some of the key developments that enable these more portable and efficient technologies are materials and fabrication, surface passivation, and improved sample handling. One important technique used in nearly any type of genetic testing is nucleic acid amplification.

The goals of this work are to investigate and improve passivation in fluorescent-analysis based microfluidic devices and to develop a lab-on-a chip device for sequence specific amplification and fluorescent detection. It incorporates helicase dependent amplification, fluorescent detection, hand operated sample manipulation, and complete USB power.

Microfluidic DNA analysis systems were improved in two ways: 1) a technique was established to passivate glass microfluidic devices for fluorescence-based assays, and 2) a portable DNA analysis device that incorporates helicase-dependent amplification and on-chip fluorescent detection was designed.

Arduino and MATLAB code for microfluidic Point of Care (POC) device

flow rate calculation in the microfluidic channels using the rectangular channel flow equations

https://arxiv.org/ftp/arxiv/papers/1703/1703.08583.pdf

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